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. 2022 Mar 7;13(3):468. doi: 10.3390/genes13030468

Figure 3.

Figure 3

miR-185-5p suppression promotes inflammation and phagocytosis. (A) The transfection efficiency in RAW264.7 macrophages with 150 nM miR-185-5p inhibitors transfections vs. nontargeting control (anti-miR NC) by RT-qPCR. (B) Gene expressions (RT-qPCR) and (C) cytokine secretions (ELISA) in LPS-induced RAW264.7 macrophages transfected with 150 nM miR-185-5p inhibitors or anti-miR NC. (D) Representative images showing phagocytosis of fluorescein-labeled E. coli bioparticles (FITC-Green, DAPI-Blue) in LPS-induced RAW264.7 macrophages with 150 nM miR-185-5p inhibitors or anti-miR NC transfection. The scale bars correspond to 100 μm. (E) Quantification of mean fluorescence intensity of phagocytosis. Mean ± SD; n = 3–6; * p < 0.05; *** p < 0.001.