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. 2022 Mar 7;13(3):468. doi: 10.3390/genes13030468

Figure 5.

Figure 5

CDC42 is a direct target of miR-185-5p. (A) The intersection of miR-185-5p downstream target genes in ENCORI, miRWalk, and miRDB. Gene expression (B) and protein level (C,E) of CDC42 in RAW264.7 macrophages 48 h after transfection of miR-185-5p mimics or miR-185-5p inhibitors and miR NC or anti-miR NC. (D,F) Quantification of protein expression in Western blots. (G) Bioinformatic prediction of miR-185-5p target sites within the CDC42 3′ UTR. (H) Dual luciferase reporter assays examined miR-185-5p target sites within the CDC42 3′ UTR in 293T cells, the relative luciferase activity was measured. Mean ± SD; n = 3–5; *** p < 0.001.