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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Matrix Biol. 2021 Nov 20;105:72–86. doi: 10.1016/j.matbio.2021.11.002

Fig. 7.

Fig. 7.

Asymmetrical immunolabeling pattern of polarity protein Par3. Par3 localization within the clusters restricted to the same pole of the cell membrane as E-cadherin. Anti-actin antibody used as the symmetrical membrane marker. Actin represented with grey pseudo color; Par3 in red, E-cadherin in yellow and nucleus in blue. (a, b) Par3 and E-cadherin colocalize (represented in orange) basal to the nucleus position indicated by white arrows and asterisks. (c, d) YZ and XZ plane sections of Alexa Fluor 555 E-cadherin channel respectively. (e, f) YZ and XZ plane sections of Alexa Fluor 647 Par-3 channel respectively. (g) three-dimensional volume rendering of the cluster viewed using all channels.