Skip to main content
. 2022 Mar 11;23(6):3030. doi: 10.3390/ijms23063030

Figure 4.

Figure 4

Confocal scanning laser microscopy findings of Aspergillus fumigatus (ASP) treated with Asian sand dust (ASD). (A–C) show the representative confocal scanning laser microscopic findings. A. fumigatus with nasal epithelial cells (Epi+ASP) at 24 h (A), 48 h (B), and 72 h ((C) 60 × magnification). The arrowhead indicates concanavalin A (Con-A) stain binding at the fungal cell wall’s polysaccharide and extracellular matrix, and the arrow indicates the FUN-1 stain binding to metabolically active fungi. When ASP was incubated with primary nasal epithelial cells, the fluorescent intensity of Con-A (D) and FUN-1 (E) increased in a time-dependent manner. The coculture of ASP and 50 μg/mL of ASD (ASD50) or 100 μg/mL of ASD (ASD100) significantly enhanced the Con-A and FUN-1 intensity at 48 h and 72 h. *: p < 0.05 compared to Epi+ASP, k = 10.