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. 2022 Mar 21;23(6):3408. doi: 10.3390/ijms23063408

Figure 4.

Figure 4

HMGCR and LDLR protein level in brain areas of Fmr1-Δexon 8 and WT male adolescent (PND35) and adult (PND90) rats. (A) illustrates a typical Western blot (top) and the densitometric analysis of HMGCR and LDLR in the amygdala of PND35 and PND90 rats. (B) illustrates a typical Western blot (top) and the densitometric analyses of HMGCR and LDLR in the prefrontal cortex (CORTEX) of PND35 and PND90 rats. (C) illustrates a typical Western blot (top) and the densitometric analyses of HMGCR and LDLR in nucleus accumbens of PND35 and PND90 rats. Tubulin served as a housekeeping protein to normalize protein loading. Values represent mean ± SD obtained from 6 animals performed in duplicate. Statistical analysis was carried out by using the unpaired Student’s t-test. * = p < 0.05 vs. WT; ** = p < 0.01 vs. WT; *** = p < 0.001 vs. WT; **** = p < 0.0001 vs. WT.