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. 2022 Feb 27;14(3):487. doi: 10.3390/v14030487

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Pathogenicity analysis of PEDV isolates in new-born piglets. (a) The severity of diarrhea was scored based on clinical examination; 0, normal and no diarrhea; 1, mild and fluidic feces; 2, moderate watery diarrhea; 3, severe watery and projectile diarrhea; 4, death. (b) Survival rate of piglets in each group. (c) Fecal virus shedding in PEDV-challenged and control groups by RT-qPCR. (d) Viral RNA shedding titers of each group were determined by RT-qPCR in the duodenum, jejunum and ileum of piglets. (e) The piglet body weights between the two PEDV-challenged groups and control groups were compared. Error bars indicate the standard deviations from each group (n = 3). * p < 0.05.

Pathogenicity analysis of PEDV isolates in new-born piglets. (a) The severity of diarrhea was scored based on clinical examination; 0, normal and no diarrhea; 1, mild and fluidic feces; 2, moderate watery diarrhea; 3, severe watery and projectile diarrhea; 4, death. (b) Survival rate of piglets in each group. (c) Fecal virus shedding in PEDV-challenged and control groups by RT-qPCR. (d) Viral RNA shedding titers of each group were determined by RT-qPCR in the duodenum, jejunum and ileum of piglets. (e) The piglet body weights between the two PEDV-challenged groups and control groups were compared. Error bars indicate the standard deviations from each group (n = 3). * p < 0.05.