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. 2022 Mar 12;14(3):586. doi: 10.3390/v14030586

Table 1.

Primers used for the generation of recombinant 301B/1.

Modification 1 Direction 2 Sequence (5′→3′) 3
UL13-K157A Forward AGCTATGGAGAAGTTAAAGTATTTAAGGGTGCAAATGTAGCCGTCGCGAAGGTGTTCGAGTGTTTTAGGGATAACAGGGTAATCGATTT
Reverse CAGTGTCATAAGCAATTCGGTCTTGAAACACTCGAACACCTTCGCGACGGCTACATTTGCACCCTGCCAGTGTTACAACCAATTAACC
UL13-A157K Forward AGCTATGGAGAAGTTAAAGTATTTAAGGGTGCAAATGTAGCCGTCAAAAAGGTGTTCGAGTGTTTTAGGGATAACAGGGTAATCGATTT
Reverse CAGTGTCATAAGCAATTCGGTCTTGAAACACTCGAACACCTTTTTGACGGCTACATTTGCACCCTGCCAGTGTTACAACCAATTAACC

1 Modification of the 301B/1 genome using two-step Red recombination. 2 Directionality of the primer. 3 Italics indicate the template-binding region of the primers for PCR amplification with pEP-KanSII. Red indicates unique upstream integration sequences. Green indicates unique downstream integration sequences. Nucleotides targeted (position 157 of UL13) for mutagenesis are underlined.