Figure 4.

Single-cell RNA-seq (scRNA-seq) helps in dealing with solid and circulating tumor tissues in cancer research. The figure shows an analysis of tissue samples taken from cancer patients by mounting them on glass slides and then tissue permeabilization on glass slides. RNA is amplified using UMIs and imaged without losing the spatial localization of RNA analytes. In the above figure, the second route shows the isolation of cells from tissue samples up to single-cell level, then cell sorting by microfluid device, and then clustering of cells according to RNA sequences performed with NGS.