Figure 8.

Amino acid sequence alignment of R. dominica aquaporins. The deduced amino acids of the R. dominica AQPs (excluding big brain) were aligned using CLUSTALW. NPA motifs are highlighted in grey. Residues that correspond to the ar/R selectivity site from Homo sapiens AQP1 (F56, H180, C189, R195; EAL24446.1) are highlighted in magenta, cyan, red, and yellow, respectively. Schematic below sequence alignment show domain structure based on predictions from the TMHMM Server v. 2.0. Rectangular boxes denote the position of the transmembrane helices (blue boxes) and the hemi-helices with the NPA motifs (green boxes). Intra- and extracellular loops A-E are denoted as ovals with letters. N^t, amino-terminal domain, C^t, carboxyl-terminal domain.