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. 2022 Mar 12;11(3):344. doi: 10.3390/pathogens11030344

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Sequence logo of an alignment of the 29 aa repeat sequences in ROM4.5 alleles of Babesia bovis isolates of different geographic origins. The alignment was generated using the twelve 29 aa slightly different repeats identified in B. bovis isolates originating from North and South America, South Africa, Australia and Israel. Red arrows mark nine polymorphic positions found in the analyzed sequences. The overall height of each position gives the grade of sequence conservation in bits, and the height of each aa in a given stack corresponds with its relative frequency. Hydrophilic aa are shown in blue; neutral aa are shown in green; and hydrophobic aa are shown in black.