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Results of annexin V/PI staining by flow cytometry analysis. For each OS cell line, the results of flow cytometry analysis are reported after 48 h of culture without any treatment (Untreated), or treated with DMSO, or with two different concentrations of RSV (60 or 120 μM). The Q2 quadrant shows the percentage of double-stained cells for annexin V/PI and apoptotic cells. DMSO is the vehicle-related control of RSV and exhibits negligible effect on OS cell lines.
