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. 2022 Mar 25;8(12):eabl5723. doi: 10.1126/sciadv.abl5723

Fig. 1. Mettl14 depletion in intestinal epithelial cells triggers a wasting phenotype and spontaneous colitis.

Fig. 1.

(A) Representative image of Mettl14 WT and Mettl14 KO mice at 2 weeks of age. (B and C) Body weight [(B), n = 5 to 8 per group] and colon length [(C), n = 6 to 10 per group] of Mettl14 WT and Mettl14 KO mice from 2 to 8 weeks. (D) Fecal water content of Mettl14 WT and Mettl14 KO mice at 2 weeks of age (n = 6 per group). (E) Survival rate of Mettl14 WT (n = 12) and Mettl14 KO (n = 14) mice was observed from 2 to 8 weeks, and the corresponding survival curves were plotted. (F) Representative image of autopsies from Mettl14 WT and Mettl14 KO colon. (G) Representative H&E staining of proximal and distal colon sections from Mettl14 WT and Mettl14 KO mice at 2, 4, and 8 weeks of age (scale bars, 100 μm). (H) Representative Muc2 staining of proximal and distal colon sections from Mettl14 WT and Mettl14 KO mice at 8 weeks of age (scale bars, 70 μm). (I and J) Quantitative analysis of H&E score [(I), n = 4 to 7 per group] and the number of goblet cells [(J), n = 10 per group]. Data are presented as means ± SD. Two-sided Student’s t test (B to D, I, and J) and log-rank (Mantel-Cox) test (E) were performed (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001).