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. 2022 Mar 8;11:e75382. doi: 10.7554/eLife.75382

Figure 5. Chromosome alignment and lamina depolymerization are prerequisites for pronuclear membranes scission.

Figure 5.

(A) Graphs showing the intercentrosomal distance normalized to embryo length in percentage in wild-type and lmn-1 8A embryos during mitosis. Times are in seconds relative to anaphase onset (0 s). The graph on the right is a zoom of the first graph focused on the 80 s before anaphase onset (0 s). (B) Graphs showing the intercentrosomal distance normalized to embryo length in percentage in lmn-1 8A embryos exposed to mock RNAi (ctrl) or klp-7(RNAi) during mitosis. Times are in seconds relative to anaphase onset (0 s). The graph on the right is a zoom of the first graph focused on the 80 s before anaphase onset (0 s). (C) Spinning disk confocal micrographs of one-cell stage embryos of the indicated genotype expressing the inner nuclear membrane protein GFP::LEM-2 (shown alone, and in green in the merged images) and mCherry::Histone (magenta, in the merged image). Times are in seconds relative to anaphase onset (0 s). The fraction of embryos that showed the presented phenotype is indicated at the bottom right of each image. All panels are at the same magnification Scale bar, 10 μm. The orange arrowheads point to the pronuclear envelopes scission event. (D) Percentage of embryos presenting zero- (green bars), one- (orange bars), or two- (red bars) paired nuclei at the two-cell stage upon exposure to mock RNAi (ctrl) or klp-7(RNAi). The number of embryos analyzed (n) is indicated on the graph and was collected from three independent experiments.

Figure 5—source data 1. Quantification of: (A) The intercentrosomal distance normalized to embryo length starting 80 s before anaphase onset (0 s) in lmn-1 wt and lmn-1 8A embryos; (B) The intercentrosomal distance normalized to embryo length starting 80s before anaphase onset (0s) in lmn-1 8A embryos upon exposure to mock (control, ctrl) or klp-7(RNAi); (E) Percentage of gfp-lem-2; lmn-1 8A embryos presenting zero-, one-, or two-paired nuclei at the two-cell stage upon exposure to mock RNAi (ctrl) or gpr-1/2(RNAi).