| Strain, strain background (Mus musculus) |
C57BL/6 |
Charles River or Jackson Labs |
Cat#JAX000664; RRID: IMSR_JAX:000664
|
|
| Antibody |
Anti-Thy1/anti-CD90(rat monoclonal) |
Thermo Fisher Scientific |
#17-0902-82 |
1:200 |
| Antibody |
Anti-L1cam(rat monoclonal) |
Miltenyi Biotec |
#130-102-243 |
1:10 |
| Antibody |
Anti-CD90 pre-conjugated (rat monoclonal) |
Miltenyi Biotec |
#130-049-101 |
1:200 |
| Antibody |
Anti-RBPMS (guinea pig polyclonal) |
PhosphoSolutions |
#1832-RBPMS |
1:1000 |
| Antibody |
Anti-KI67 (rabbit monoclonal) |
Thermo Fisher Scientific |
#MA5-14520 |
1:250 |
| Chemical compound, drug |
Fluoro-Gel |
Electron Microscopy Sciences |
#17985 |
|
| Commercial assay or kit |
MACS Large Cell Columns |
Miltenyi Biotec |
#130-042-202 |
|
| Sequence-based reagent |
Drop-seq beads |
ChemGenes Corporation |
#Macosko201110 |
|
| Commercial assay or kit |
Papain dissociation system |
Worthington |
#LK003160
|
|
| Commercial assay or kit |
Chromium Single Cell 30Library and Gel Bead Kit v2, 10X Genomics 16rxns |
10X Genomics |
#120237 |
|
| Software, algorithm |
Cell Ranger v2.6.0 |
10X Genomics |
https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest
|
|
| Software, algorithm |
ImageJ (Fiji) version 2.1.0 |
Fiji |
https://imagej.net/Fiji
|
|
| Software, algorithm |
R 3.6.2 |
The R Foundation |
https://www.r-project.org/
|
|
| Software, algorithm |
RStudio 1.3.1056 |
RStudio |
https://www.adobe.com
|
|
