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. 2022 Mar 23;14(1):2056291. doi: 10.1080/20002297.2022.2056291

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Effect of NAM on S. mutans biofilm. (a) Effect of NAM on S. mutans biofilm formation assessed by measuring the number of CFU in the 6 h, 12 h and 24 h biofilm. (b) Three-dimensional visualization and double-labeling imaging of 6 h biofilms of S. mutans formed on glass coverslips under NAM treatment. Live bacteria and EPS were green-labeled and red-labeled, respectively. (c) and (d) Quantification of the amounts of EPS and bacteria in each scanned layer of 6 h biofilms of S. mutans. The biomass of bacteria (c) and EPS (d) in each group. Values represent the means and standard deviations from three independent experiments (*P < 0.05, **P < 0.01, ****P < 0.0001 compared with the untreated control).