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. 2022 Mar 23;29(1):937–949. doi: 10.1080/10717544.2022.2053762

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — ND-DOX-loaded platelets maintained viability probably through induction of autophagy. (A) Microscopy of FDA staining of platelets loaded with ND-DOX or DOX. (B) Viability of platelets loaded with ND-DOX or DOX, assayed by CCK-8 test. (C–E) Western blot analysis of caspase-3, ATG5, Beclin-1 and LC-3 expression in platelets loaded with ND-DOX or DOX. (F) TEM observation of platelets loaded with ND-DOX (Plt@ND-DOX). White arrow marked was autophagosomes. Values were means ± SD (n = 3, * p < .05). Plt@ND-DOX refers to platelets loaded with ND-DOX.