Fig. 5.

RSV reduces LX-2 cell inflammation by inhibiting the NF-κB pathway in vitro. LX-2 cells were treated with or without RSV (10, 20, or 50 µM) and stimulated with TGF-β for 24 h. mRNA levels and supernatant levels of TNF-α and IL-6 were measured by qPCR (A) and ELISA (B). C Levels of p-p65-NF-κB, p65-NF-κB, p-IκBα, IκBα, and β-actin were measured by western blot. Representative images were shown on the left and summarized results are on the right. A–C Results from three independent experiments are shown as mean ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001.