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. 2022 Mar 25;13:1590. doi: 10.1038/s41467-022-28423-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Workflow overview. b Overview of automated experimental pipeline. Scale bar: 35 μm. Running this pipeline yielded low variation across batches in: c well-level cell count; d well-level foreground staining intensity distribution per channel and plate; and e well-level image focus across the endoplasmic reticulum (ER) channel per plate, for n = 96 wells per plate. Box plot components are: horizontal line, median; box, interquartile range; whiskers, 1.5× interquartile range; black squares, outliers.