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. 2022 Mar 25;13:1606. doi: 10.1038/s41467-022-29205-8

Fig. 2. Candidate gene overexpression mediates resistance in other cell types and in vivo.

Fig. 2

a Cell survival against ESO T cell cytotoxicity of H1793 (NY-ESO-1+, HLA-A2−) non-small cell lung adenocarcinoma transduced with HLA-A2 and ORFs encoding candidate genes. N = 12. Two-tailed t tests with adjustments for multiple comparisons were performed. b Heatmap summarizing results from ESO T cell cytotoxicity assays for eight cell lines derived from different tissues. Each value represents the significance of the difference between the survival of each ORF and GFP control. Two-tailed t tests with adjustments for multiple comparisons were performed. c Schematic of the in vivo experiments to test the response of A375 xenografts overexpressing candidate genes to adoptive cell transfer (ACT) in NSG mice. d, e Tumor growth in mice receiving ACT of ESO T cells. Data are representative of two independent experiments. N = 12. d Tumor volume is shown. Two-tailed t tests with adjustments for multiple comparisons were performed. e Overall survival is shown. Mantel–Cox log-rank tests were performed. All values are mean ± s.e.m. Source data are provided in Source Data 2.