Table 3.
Selected differential expressed P. pinaster miRNAs and predicted target genes.
| miRNA | Expression pattern | Log2FC | Target ID | Target annotation | GO terms/pathways |
|---|---|---|---|---|---|
| DE miRNAs inoculated vs control | |||||
| ppi-miR166f | Downregulated | − 0.623 | unigene8322 | Protein TIFY 6B-like | Plant hormone signal transduction |
| unigene942 | Protein TIFY 6B | Plant hormone signal transduction | |||
| ppi-miR947e | Downregulated | − 1.214 | unigene105220 | Protein TIFY 10A | Plant hormone signal transduction |
| ppi-miRnovel43f | Downregulated | − 1.541 | unigene26097 | Transcription factor MYC4-like | Plant hormone signal transduction |
| ppi-miR390b | Downregulated | − 2.065 | unigene3146 | Nematode resistance protein-like HSPRO1 | Defence response |
| ppi-miR11565a-i | Upregulated | 3.734 (± 1.83) | isotig42180 | WRKY transcription factor 20 | Plant–pathogen interaction |
| unigene650 | WRKY transcription factor 44 | Plant–pathogen interaction | |||
| ppi-miRnovel816 | Upregulated | 1.754 | isotig42166 | Calcium-dependent protein kinase 28 (CPK28) | Plant–pathogen interaction |
| ppi-miR11565h | Upregulated | 5.948 | unigene12702 | Disease resistance RPP13-like protein 4 | Plant–pathogen interaction |
| ppi-miR11458e | Upregulated | 6.313 | isotig49219 | Disease resistance protein At1g61300 | Plant–pathogen interaction |
| ppi-miR11458f | Upregulated | 5.155 | unigene57660 | Disease resistance protein RPS2 | Plant–pathogen interaction |
| ppi-miR3627u | Upregulated | 5.053 | isotig51344 | Disease resistance protein RPS2-like | Plant–pathogen interaction |
| ppi-miR529l | Upregulated | 5.121 | unigene116482 | Probable RLK | Plant–pathogen interaction |
| ppi-miR946j | Upregulated | 5.178 | isotig75044 | Disease resistance RPP13-like protein 4 | Plant–pathogen interaction |
| ppi-miR396j | Upregulated | 4.923 | unigene75931 | Disease resistance protein At4g27190-like | Plant–pathogen interaction |
| isotig42452 | Bifunctional levopimaradiene synthase (LPS) | Diterpenoid biosynthesis | |||
| Novel_1871 | Upregulated | 1.887 | unigene31062 | Bifunctional abietadiene synthase (AS) | Diterpenoid biosynthesis |
| ppi-miR11436b, f-k, m | Upregulated | 2.428 (± 1.86) | unigene2998 | Bifunctional abietadiene synthase (AS) | Diterpenoid biosynthesis |
| Upregulated | unigene9633 | Bifunctional abietadiene synthase (AS) | Diterpenoid biosynthesis | ||
| ppi-miR11436b, f-m | Upregulated | 2.754 (± 2.00) | isotig44195 | 4-Hydroxy-3-methylbut-2-enyl diphosphate reductase | Terpenoid backbone biosynthesis |
| ppi-miRnovel1251 | Upregulated | 4.890 | unigene97227 | Bifunctional levopimaradiene synthase (LPS) | Monoterpenoid biosynthesis |
| ppi-miR3627s | Upregulated | 5.761 | isotig56835 | Short-chain dehydrogenase/reductase 2b-like | Monoterpenoid biosynthesis |
| ppi-miR11436b, f, g, j, k, m | Upregulated | 2.725 (± 2.11) | isotig34808 | Peroxiredoxin Q | Cell redox homeostasis |
| ppi-miR1314f | Upregulated | 5.123 | isotig25066 | Peroxiredoxin-2E | Cell redox homeostasis |
| ppi-miR3627l | Upregulated | 3.980 | isotig25066 | Peroxiredoxin-2E | Cell redox homeostasis |
| ppi-miR529c, y | Upregulated | 4.383 (± 2.91) | isotig12834 | Thioredoxin F-type | Cell redox homeostasis |
| DE miRNAs resistance vs susceptible | |||||
| ppi-miR166h | R > S | 6.418 | unigene67614 | Putative RLK | Protein serine/threonine kinase activity |
| ppi-miR951f | R < S | − 1.329 | unigene93826 | Putative RLK | Protein serine/threonine kinase activity |
| unigene5558 | Putative RLK | Protein kinase activity | |||
| ppi-miR947f | R > S | 5.163 | isotig45349 | GDP-L-fucose synthase 2 | Amino sugar and nucleotide sugar metabolism |
| Novel_110 | R < S | − 1.550 | isotig51371 | Protein COBRA-like | Cellulose microfibril organization |
| unigene925 | Ninja family protein | Signal transduction | |||
| ppi-miR3627m | R < S | − 3.661 | unigene82871 | Short-chain dehydrogenase reductase 2a-like | Oxidoreductase activity |