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. 2022 Mar 25;12:5196. doi: 10.1038/s41598-022-09074-3

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© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Longitudinal behavioral analysis of Ndufs2^+/- and Ndufs2^+/+ mice. (a) Novel object recognition test total exploration time. Male and female data combined. n = 7–25 per group, multiple t-tests not significant. (b) Novel object recognition test discrimination index. Male and female data combined. n = 7–25 per group, multiple t-tests not significant. (c) Female forced maximal exercise on accelerating treadmill challenge total time on treadmill. n = 4–8. Multiple t-tests not significant. (d) Male forced maximal exercise on accelerating treadmill challenge total time on treadmill. n = 2–6. Multiple t-tests not significant. (e) Female day 1 accelerating rotarod challenge latency to fall. n = 4–13. Multiple t-tests not significant. (f) Male day 1 accelerating rotarod challenge latency to fall. n = 5–18. Multiple t-tests not significant. (g) Female day 4 accelerating rotarod challenge latency to fall. n = 4–13. Multiple t-tests not significant. (h) Male day 4 accelerating rotarod challenge latency to fall. n = 5–18. Multiple t-tests not significant. (i) Female open field test total distance. n = 4–13. Multiple t-tests not significant. (j) Male open field test total distance. n = 5–18. Multiple t-tests not significant. (k) Female grip strength. n = 4–13. Multiple t-tests not significant. (l) Male grip strength. n = 5–18. Multiple t-tests not significant. (a–l) Multiple t-tests refers to adjusted t-tests with a two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli, with Q = 1% Ndufs2^+/+ vs Ndufs2^+/− independently for each time point. n.s. not significant by genotype.