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. 2022 Mar 25;13(3):270. doi: 10.1038/s41419-022-04728-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A The lncRNAs that potentially bind to RBM47 predicted by bioinformatics software. B The relative mRNA expression of related lncRNAs detected by qRT-PCR in TPC1 cells with RBM47 knockdown. C The enrichment of SNHG5 expression in anti-RBM47 precipitates shown by RIP assay. D The half-life of SNHG5 in TPC1 cells treated with RBM47 knockdown. E SNHG5 expression in PTC tissues and normal tissues from TCGA database. F The relative mRNA expression of SNHG5 in 100 paired PTC tissues and adjacent non-cancerous tissues. G The fold change of SNHG5 expression in 100 PTC tissues. H The relative mRNA expression of SNHG5 in PTC cells and normal thyroid cells measured by qRT-PCR. I The correlation between RBM47 and SNHG5 in PTC tissues. J The localization of GAS8-AS1 in cytoplasm verified by FISH. Scale bar = 20 μm. K, L Proliferation of SNHG5-silenced or -overexpressed cells evaluated by CCK-8 assay and plate colony assay. M–O LC3 II/LC3 I ratio detected by WB, LC3 puncta evaluated by IF and autophagosomes shown by TEM (red arrow) in PTC cells with SNHG5 upregulation. Scale bar in TEM = 5 μm (Upper) and 1 μm (Below), scale bar in IF = 20 μm. Statistical differences were analyzed using the independent samples t-test; data are shown as the mean ± standard error of the mean based on three independent experiments. *p < 0.05, **p < 0.01.