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. 2022 Mar 25;12:5215. doi: 10.1038/s41598-022-09211-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Decreased levels of fOCD variant aggrecan protein in patient cartilage. Cartilage tissue explants from a patient heterozygous for the fOCD-linked p.V2455M ACAN variant were obtained at arthroplasty. Some explants were treated with interleukin-1 and oncostatin M to stimulate protein production. After explant culture, the tissue explants were homogenized and extracted, and conditioned medium was collected. After GluC digestion, wild type and p.V2455M variant-specific peptides were quantified by mass spectrometry (MRM LC-MSMS) in the explant extracts (a) and medium (b). Note the decreased levels of p.V2455M variant compared to wild-type aggrecan proteoglycan in explant tissue (a) as well as in the culture medium (b).