Fig. 3.

Post-translational modifications (PTM) of histones in spermatozoa of control or treated mice. a H3K4me3, b H3K27me3, c H3K9me2/3, d H3K4me2, e H3K9me, f H4K12ac. g Representative images of the localization of the positive marks (green) of PTM of histones in sperm of control and treated mice. The sperm nucleus is labeled with DAPI (blue). Scale bar, 10 μm. Data were analyzed with Student’s t test for parametric data or Mann–Whitney U tests for non-parametric data. *p < 0.05. n = 10 males per group. The arrows indicate the positive mark inside the sperm