Fig. 7.

Testicular histology in the progeny (F1). a Cross-section images representative of testicular morphology in control (left panel) and treated (right panel) groups. Stained with Hematoxylin and Eosin. Original magnification of images: 20 × up, 100 × middle, 400 × down (scale bar: 500 μm, 100 μm, and 25 μm, respectively). b–d) Morphometric analysis of the testes: diameters of seminiferous tubules (b), their lumens (c), and the thickness of the seminiferous tubule epithelium (d). At least 50 tubules were evaluated for each male, n = 3 litter per group (3/4 males for each) Nested t test. *p < 0.05, **p < 0.01. e Daily sperm production per testis (DSP) and f efficiency (DSP per gram of testis) of sperm production in control and treated group, n = 5 litter per group (2/3 males for each). Nested t test. *p < 0.05