REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
GST-Tag Antibody	Affinity Biosciences	Cat# T0007; RRID: AB_2839414
Anti-MBP Monoclonal Antibody	New England Biolabs	Cat# E8032S; RRID: AB_1559730
Bacterial and virus strains
DH5α competent cell	Vazyme	Cat# C502-02
BL21 (DE3) competent cell	Vazyme	Cat# C504-02
Rosetta (DE3) competent cell	TOLOBIO	Cat# CC96139-01
Chemicals, peptides, and recombinant proteins
Sodium hypochlorite	SINOPHARM	Cat# 80010428
Triton X-100	Sigma-Aldrich	Cat# V900502
Murashige and Skoog Basal Medium (MS)	Sigma-Aldrich	Cat# M5519
Sucrose	Sigma-Aldrich	Cat# V900116-500G
Agar	Sigma-Aldrich	Cat# A1296-1KG
Phos-tag Acrylamide	Wako	Cat# 304-93521
Manganese (II) chloride tetrahydrate (MnCl2)	Sigma-Aldrich	Cat# V900197-500G
Sodium dodecyl sulfate	Sigma-Aldrich	Cat# V900859-500G
Ammonium persulfate substitute (APS substitute)	Beyotime	Cat# ST005-10g
Glycine	Sigma-Aldrich	Cat# V900144-5KG
Methanol	SINOPHARM	Cat# 10014118
Ethylenediaminetetraacetic acid (EDTA)	Sigma-Aldrich	Cat# V900106-500G
Glutathione Sepharose beads	Cytiva	Cat# 17075601
Amylose resin	New England Biolabs	Cat# E8021S
Phenylmethylsulphonyl fluoride (PMSF)	Sigma-Aldrich	Cat# P7626
L-Glutathione reduced	Sigma-Aldrich	Cat# V900436-25G
D-(+)-Maltose monohydrate	Sigma-Aldrich	Cat# V900435-100G
Trizma base	Sigma-Aldrich	Cat# V900483-5KG
Magnesium chloride hexahydrate (MgCl2)	Sigma-Aldrich	Cat# V900020-500G
dithiothreitol (DTT)	Beyotime	Cat# ST040-5g
adenosine triphosphate (ATP)	Coolaber	Cat# CA1261-5G
L-Kynurenine (Kyn)	Sigma-Aldrich	Cat# K8625
TG-003	Sigma-Aldrich	Cat# T5575
4-Phenoxyphenylboronic acid (PPBo)	Sigma-Aldrich	Cat# 480142
TRIzol	Invitrogen	Cat# 15596018
Critical commercial assays
Mut Express MultiS Fast Mutagenesis Kit V2	Vazyme	Cat# C215-01
Deposited data
RNA-seq dataset	National Genomics Data Center (https://ngdc.cncb.ac.cn)	CRA001128 and CRA003933
The Arabidopsis genome	TAIR10	http://ensemblgenomes.org
Experimental models: Organisms/strains
Arabidopsis afc2-1	SALK_118114	N/A
Arabidopsis afc2-2	SALK_043601	N/A
Arabidopsis pif4-2	Ma et al., 2016	N/A
Arabidopsis afc2-1 pif4-2	This paper	N/A
Arabidopsis afc2-2 pif4-2	This paper	N/A
Oligonucleotides
GST-AFC2 Forward: TGGGATCCCCGAATTCATGGAGCGT GTGCATGAATTC	This paper	N/A
GST-AFC2 Reverse: GTCGACCCGGGAATTCTCTTCTCCTTGCGAAAAAC	This paper	N/A
MBP-RSZ21 Forward: TATCGTCGACGGATCCATGACGAGGGTTTATGTC	This paper	N/A
MBP-RSZ21 Reverse: CAGGGAATTCGGATCCCACCCCATTGGCATATGG	This paper	N/A
GST-AFC2R284K Forward: ACCaaaCATTATAGGGCACCAGAAGTCATTTT	This paper	N/A
GST-AFC2R284K Reverse: GCCCTATAATGtttGGTTGATACAATGTAGGTTTGGTCC	This paper	N/A
GST-AFC2H285K Forward: ACCAGAaaaTATAGGGCACCAGAAGTCATTTTAGG	This paper	N/A
GST-AFC2H285K Reverse: GCCCTATAtttTCTGGTTGATACAATGTAGGTTTGG	This paper	N/A
Recombinant DNA
GST-AFC2	This paper	N/A
MBP-RSZ21	This paper	N/A
GST-AFC2R284K	This paper	N/A
GST-AFC2H285K	This paper	N/A
Software and algorithms
ImageJ	NIH	http://rsb.info/nih/gov/ij