TBS, pH 7.6

Reagent	Final concentration	Amount
NaCl	150 mM	8.766 g
Tris base	50 mM	6.057 g
HCl, 6 M	n/a	To pH 7.6
ddH2O	n/a	1 L
Total	n/a	1 L

50× wash buffer and 10× target retrieval solution are supplied in the RNAscope Multiplex Fluorescent Reagent Kit v2, ACD, #323100.

RNAse-free reagents are not required for this protocol.

All buffers are stored at room temperature (RT; in our lab 20°C–21°C) for up to one year.