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. 2022 Mar 22;27:100783. doi: 10.1016/j.neo.2022.100783

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© 2022 The Authors. Published by Elsevier Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 6 — Activation of STAT3 and increased expression of its target genes by DEC1 in DLD1 cells. (A-E) The overexpression of DEC1 increased the protein levels of p-STAT3/STAT3(t-text, t=25.82, df=4, p<0.0001), survivin (t-text, t=6.132, df=4, p=0.0036), Bcl2(t-text, t=4.953, df=4, p=0.0077) and cyclin D1(t-text, t=5.653, df=4, p=0.0048). (F, G) The p-STAT3/STAT3 level induced by IL-6 (two-way ANOVA, gene: F (1, 12)=311.5, p<0.0001, IL-6: F (2, 12)=320.4, p<0.0001, interaction: F (2, 12)=62.18, p<0.0001) in the DEC1 overexpression cells. (H–L) The knockdown of DEC1 decreased the protein levels of p-STAT3/STAT3 (t-text, t=10.36, df=4, p=0.0005), survivin (t-text, t=4.368, df=4, p=0.0120), Bcl2 (t-text, t=9.589, df=4, p=0.0007), and cyclin D1 (t-text, t=6.604, df=4, p=0.0027). (M, N) The p-STAT3/STAT3 level induced by stattic (two-way ANOVA, gene: F (1, 12)=365.7, p<0.0001, IL-6: F (2, 12)=511.5, p<0.0001, interaction: F (2, 12)=72.79, p<0.0001) in the DEC1 knockdown stable cells. All the experiments were repeated at least three times, and the data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control (PBS) or 0 min, and ### p < 0.001 vs. the corresponding time of Flag-CMV2 or LV-CON.