Fig. 8.

Example application of the characterisation of the dose–response to MWCNT with BMDx. The preprocessed data were downloaded from eUTOPIA in a format compatible with the BMDx input. After completing the benchmark dose analysis, the results can be explored via various visual presentations. For example, (A) the distributions of the computed BMD values were compared between the time points. The BMD values computed at 24 h of exposure exhibit a higher peak at low doses compared to the later time points. (B) the Venn diagram indicates a larger number of dose-dependent genes at 24 h than at 48 and 72 h. (C) The best model for TNF with the computed BMD (blue), BMDL (red), BMDU (green) and IC/EC50 (green) values. (D) Selected pathways enriched in the functional enrichment indicate that the mean BMD values for distinct biological functions increase at later time points. The colour of the cell represents the mean BMD values of the genes enriching the pathway. (E) Line graph representing the genes enriching TNF signalling pathway at 48 h with their BMD, BMDL and BMDU values plotted.