Figure 4.

Effects of Miat on podocyte mitotic catastrophe are mediated by Sox4 in vitro

(A) Flow cytometry showed the apoptosis rate of podocytes (n = 4). (B) Flow cytometry illustrated the cell-cycle progression of podocytes (n = 3). (C) Immunostaining of mitosis with antibodies against α-tubulin (green; to indicate mitotic spindles) and histone-3 phosphorylated at serine 10 (H3-Ser10) (red; to indicate metaphase) (n = 3). Scale bar, 50 μm. (D) The percentages of podocytes with normal or abnormal mitotic processes were monitored and analyzed (n = 3). Scale bar, 10 μm. (E) Western blotting depicted the expression of Sox4, p53, p21^cip1/waf1, CyclinB, and cdc2 (n = 3). (F–J) Levels of the Sox4 (n = 3), p53 (n = 5), p21^cip1/waf1 (n = 4), CyclinB (n = 4), and cdc2 (n = 3) mRNAs. Error bars represent ±SD. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.