Figure 3.

RP_ostability in associated with Fdx sensitivity in vivo.A, representative gels of three nucleotide transcripts produced by M. tuberculosis RNAP-σ^A alone or in complex with RbpA_Mtb^WT, RbpA_Mtb^72–111, RbpA_Mtb^R10/E17A, or RbpA_Mtb^R88A from a plasmid DNA template containing positions −39 to +4 of M. tuberculosis rrnAP3 relative to the +1 transcription start site. B, ratio of transcript produced as compared to the average of “No Factor” replicates included on the same gel. Results are plotted as individual values with the mean ± SD shown. Statistical significance of differences was determined by ANOVA and Tukey’s multiple comparison test. ‘ns’, not significant; ∗∗p < 0.01; ∗∗∗∗p < 0.0001. C, zones of inhibition (ZOI) by Fdx on bacterial lawns of M. smegmatis expressing CarD_Mtb^WT or CarD_Mtb^R25E as the only copy of carD. D, mean radii of ZOI ± SD from at least two experiments with at least three replicates at 100 μM, 250 μM, and 500 μM Fdx is plotted. Statistical significance was analyzed by two-tailed Welch’s t test. ∗p < 0.05; ∗∗p < 0.01. Fdx, fidaxomicin; RbpA, RNA polymerase binding protein A; RP_o, RNAP-promoter open complex.