Table 1.

Summary of the effects of RbpA and CarD mutants on fidaxomicin (Fdx) sensitivity and open complex (RP_o) stability, compared to WT protein

RbpA construct	In vitro Fdx sensitivity	In vivo Fdx sensitivity	RPo stability
RbpA72–111	Decrease	Decrease	Increase
RbpAR88A	No change	Increase	Decrease
RbpAR10A/E17A	Decreasea	No change	No change
CarDR25E	N/A	Increase	Decrease

^aThe level of decrease in Fdx sensitivity in vitro with RbpA^R10A/E17A is intermediate to that of RbpA^72–111, when both are compared to RbpA^WT.