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. 2022 Mar 9;28:175–189. doi: 10.1016/j.omtn.2022.03.007

Figure 1.

Figure 1

Doxorubicin-induced cardiac fibrosis and impaired heart function were exacerbated by circNlgn expression

(A) Left, circNlgn transgenic mice, circNlgn (+), showed decreased left-ventricular ejection fraction (LVEF) relative to the litter-matched negative mice, circNlgn (−), after doxorubicin treatment. ∗∗p < 0.01 versus circNlgn (−) (n = 10). Right, circNlgn (+) mice showed decreased left-ventricular fractional shortening (LVFS) compared with circNlgn (−) after doxorubicin treatment. ∗∗p < 0.01 versus circNlgn (−) (n = 10). (B) circNlgn (+) mice displayed lower left-ventricular pressure (dp/dt) than circNlgn (−) mice after doxorubicin treatment. ∗∗p < 0.01 versus circNlgn (−) (n = 10). (C) circNlgn (+) mice displayed higher heart weight/tibia length (left) and heart weight/body weight (right) than circNlgn (−) mice after doxorubicin treatment. ∗∗p < 0.01 versus circNlgn (−) (n = 10). (D) Representative echocardiography photographs showing heart function of circNlgn (+) and circNlgn (−) mice with or without doxorubicin treatment.

(E) Representative H&E staining of cross sections of heart tissues. circNlgn (+) mice displayed enlarged heart chamber. (F) circNlgn (+) mouse hearts displayed increased Masson’s trichrome (left) and Sirius red (right) staining compared with circNlgn (−) controls. Doxorubicin treatment significantly increased Masson’s trichrome and Sirius red staining in mouse hearts. ∗p < 0.05, ∗∗p < 0.01 (n = 10). (G) Representative Masson’s trichrome and Sirius red staining of cross sections of heart tissues. circNlgn (+) mouse hearts displayed increased Masson’s trichrome and Sirius red staining after doxorubicin treatment. (H) circNlgn (+) mouse hearts expressed higher levels of circNlgn than circNlgn (−) controls measured by real-time PCR. Doxorubicin treatment promoted circNlgn expression in mouse hearts. ∗∗p < 0.01 (n = 10). It is not necessary to start a new paragraph between (E) and (D). It may be good to prinit this figure in one page. I have provided a new figure (condensed a bit), so that the legend and the figure can be prinited in one page.