Figure 4.

Upregulation of Gadd45b, Sema4C, and RAD50 in circNlgn transgenic heart

(A) Volcano analysis of 24,283 mRNAs detected by RNA sequencing. Approximately 3% of the RNAs were differentially expressed. (B) Heatmap analysis showing the differentially expressed RNAs. (C) From the 307 differentially expressed mRNAs, the most up- and downregulated were selected. (D) PCR showed that PCMs from the hearts of the circNlgn-transgenic mice expressed significantly higher levels of circNlgn, Gadd45b, Sema4C, RAD50, IL-1b, IL-1rap, IL-2Rb, IL-6, EGR1, and EGR3. ∗p < 0.05, ∗∗p < 0.01 versus wild type (n = 4). (E) PCR showed that circNlgn (+) mouse heart tissues expressed high levels of circNlgn, Gadd45b, Sema4C, RAD50, IL-1b, IL-2Rb, IL-6, EGR1, and EGR3, which were further enhanced by delivery of doxorubicin. ∗∗p < 0.01 (n = 4).