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. 2022 Mar 9;28:175–189. doi: 10.1016/j.omtn.2022.03.007

Figure 5.

Figure 5

Dissecting out the downstream signal molecules of γH2AX

(A) PCR showed that PCMs transfected with circNlgn siRNA repressed expression of circNlgn, Gadd45b, Sema4C, RAD50, IL-1b, IL-2Rb, IL-6, EGR1, and EGR3. IL-1rap did not show downregulation upon circNlgn silencing. ∗p < 0.05, ∗∗p < 0.01 versus oligo (n = 4). (B) Silencing EGR1 (left) and EGR3 (right) did not affect expression of any other molecules. ∗∗p < 0.01 versus oligo (n = 4). (C and D) Silencing IL-1b decreased expression of EGR3 (C), while silencing IL-2Rb decreased expression of EGR1 (D). ∗∗p < 0.01 versus oligo (n = 4). (E) Silencing IL-6 decreased expression of EGR1 and EGR3. ∗p < 0.05, ∗∗p < 0.01 versus oligo (n = 4). (F and G) Silencing Gadd45b (F) or Sema4C (G) repressed expression of all these molecules (IL-1b, IL-2Rb, IL-6, EGR1, and EGR3). ∗p < 0.05, ∗∗p < 0.01 versus oligo (n = 4). (H) Silencing RAD50 did not affect expression of any molecules tested. ∗∗p < 0.01 versus oligo (n = 4).