Skip to main content
. 2022 Mar 9;28:175–189. doi: 10.1016/j.omtn.2022.03.007

Figure 6.

Figure 6

Effects of the signaling molecules in mediating cellular functions of circNlgn

(A) Western blot showed that circNlgn (+) heart tissues expressed high levels of Nlgn173, γH2AX, pJNK, and p38, which were further promoted by delivery of doxorubicin. (B) PCMs were transfected with circNlgn siRNAs and cultured with basal medium with 0.2 μM doxorubicin for 36 h. Silencing circNlgn suppressed Nlgn173, γH2AX, pJNK, and p38 expression. (C) Control vector- and circNlgn-transfected cells were treated with or without 0.25 μM doxorubicin for 24 h, lysed, and subjected to western blotting. circNlgn transfection increased the levels of Nlgn173, γH2AX, pJNK, and p38, which were further promoted by doxorubicin treatment. (D) Signaling pathway of circNlgn for mediating doxorubicin effect on cardiac remodeling, fibrosis, and cardiomyopathy. (E) Western blot showed that circNlgn (+) PCMs expressed high levels of γH2AX, pJNK, and p38. Silencing Gadd45b prevented circNlgn-enhanced γH2AX, pJNK, and p38. Silencing Sema4C prevented circNlgn-enhanced γH2AX and p38, but not pJNK.