Figure 1.
The effect of collagen density and oxygen tension on AMSC mesenchymal progenitor and osteochondrogenic gene expression
AMSCs were cultured in either 2D (tissue culture plastic) or 3D (0.2% and 10%) collagen type I gels for a period of 7, 14, and 21 days in either normoxic (21% O2) or hypoxic (5% O2) conditions. Gene expression markers were assessed using qPCR (Data are presented as mean ±S.E.M, ssstatistical analysis performed using one-way analysis of variance, uncorrected Fisher’s least significant difference; ∗∗∗p < 0.001; ∗∗p < 0.01; ∗p < 0.05; n = 3).