REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Recombinant Anti-CD73 antibody [EPR6114]	Abcam, Cambridge, UK	Abcam Cat# ab124725, RRID:AB_10976033
Anti-CD164 (H-4) monoclonal antibody	Santa Cruz Biotechnology, TX, USA	Santa Cruz Biotechnology Cat# sc-271179, RRID:AB_10613973
Anti-PDPN (B-11) monoclonal antibody	Santa Cruz Biotechnology, TX, USA	Santa Cruz Biotechnology Cat# sc-166906, RRID:AB_10610482
VectaShield Antifade mounting medium containing DAPI	Vector Laboratories, Burlingame, CA, USA	Vector Laboratories Cat# H-1200, RRID:AB_2336790
Podoplanin Monoclonal Antibody (NZ-1.3), PE, eBioscience™	Thermo Fisher Scientific, Loughborough, Leicestershire, UK	Thermo Fisher Scientific Cat# 12-9381-41, RRID:AB_1582263
CD73 Monoclonal Antibody (AD2), FITC, eBioscience™	Thermo Fisher Scientific, Loughborough, Leicestershire, UK	Thermo Fisher Scientific Cat# 11-0739-42, RRID:AB_10596508
CD146 Monoclonal Antibody (P1H12), FITC, eBioscience™	Thermo Fisher Scientific, Loughborough, Leicestershire, UK	Thermo Fisher Scientific Cat# 11-1469-42, RRID:AB_2043805
Chemicals, peptides, and recombinant proteins
Recombinant Human TGF-β1 (HEK293 derived)	PeproTech, London, UK	Cat#100-21
Dexamethasone	Sigma, UK	Cat#D4902
L-Proline	Sigma, UK	Cat#P5607
L-ascorbic acid 2-phosphate	Sigma, UK	Cat#A8960
3-Isobutyl-1-methylxanthine	Sigma, UK	Cat#5879
Sodium hydroxide	Sigma, UK	Cat#S5881
β-Glycerophosphate disodium salt hydrate	Sigma, UK	Cat#G9422
Rat-tail collagen type I (2.05 mg/ml in 0.6% acetic acid)	First Link (Ltd), UK	Cat#60-30-810
Critical commercial assays
RNeasy Mini kit	Qiagen, Manchester, UK	Cat#74106
High-Capacity cDNA Reverse Transcriptase Kit	Thermo Fisher Scientific, Loughborough, Leicestershire, UK	Cat#368814
iTaq Universal SYBR Green Super mix	Bio-Rad, Hertfordshire, UK	Cat#1725124
LIVE/DEAD™ reagents	Theermo Fisher Scientific, Loughborough, Leicestershire, UK	Cat#L-3224
Real Architecture For 3D Tissues (RAFT™)	Lonza Bioscience	Cat#016-1R16
Experimental models: Cell lines
hTERT-immortalised human (female) AMSCs	ATCC, MNZ, VA, USA	ATCC Cat# SCRC-4000,RRID:CVCLU602, MNZ
Oligonucleotides
Primers for CD146 Forward: GGAAGGT GTGGGTGAAAGAG	This paper	N/A
Primers for CD146 Reverse: GGACATTC AGGGTGCTCAG	This paper	N/A
Primers for CD164 Forward: CCTTAGCT TTCTCCCGAACG	This paper	N/A
Primers for CD164 Reverse: TGCTGGG TCGTGTTCTTG	This paper	N/A
Primers for CD73 Forward: ACTGGGAC ATTCGGGTTTTG	This paper	N/A
Primers for CD73 Reverse: CTCTTTGGA AGGTGGATTGC	This paper	N/A
Primers for FABP4 Forward: CATACTG GGCCAGGAATTTG	This paper	N/A
Primers for FABP4 Reverse: GGACAC CCCCTCTAAGGTT	This paper	N/A
Primers for GAPDH, NES, PDPN, PRX1, RUNX2, SOX9, TAZ, YAP, see Table S1	This paper	N/A
Software and algorithms
FlowJo™ software	FlowJo, LLC	FlowJo, RRID:SCR_008520
ImageJ	ImageJ	National Institute of Health, RRID:SCR_003070 (https://imagej.nih.gov/ij/)
GraphPad Prism version 6.0f for windows	GraphPad Prism Software, La Jolla, CA, USA	http://www.graphpad.com