Figure 2.

The CD73⁺CD104⁺population is depleted in the esophagus of active EoE patients and a murine model of EoE. (A) Immunofluorescence staining for CD73 and CD104 on biopsy specimens from non-EoE controls and patients with active EoE. Representative images are shown. Dotted line represents the basement membrane. Scale bars: 100 μm. (B–E) Biopsy specimens from non-EoE controls (n = 10), and patients with active (n = 10) or inactive EoE (n = 10) were dissociated for flow cytometry. The representative flow cytometry plots are shown in panel B. (C) The percentage of CD73⁺CD104⁺ or CD73^-CD104⁺ cells in non-EoE controls (n = 10), and patients with active (n = 10) or inactive EoE (n = 10). (D and E) Pearson correlation coefficient analyses comparing the percentage of CD73⁺CD104⁺ cells detected by flow cytometry and the number of eosinophils (eos/hpf) in (D) H&E-stained biopsy specimens or (E) CD45⁺ cells detected by flow cytometry. (F and G) After BALB/c mice were treated with the EoE protocol, their esophagi were resected for histologic sections or flow cytometry. (F) Histologic sections (H&E staining) of the esophagus from control (nontreated [NT]) or EoE protocol–treated mice. Arrowheads identify tissue-infiltrating eosinophils. (G) The percentage of CD73⁺ cells in esophageal epithelium of EoE mice (n = 3), compared with NT mice (n = 3). The data are shown as means ± SD. A 2-tailed Student t test or analysis of variance was performed for comparing 2 or multiple comparisons, respectively. ∗P < .05.