REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Primary human BDNF	Santa Cruz Biotechnology	Cat#sc-546, RRID: AB_630940
α-tubulin	Sigma-Aldrich	Cat#T5168, AB_477579
Chemicals, peptides, and recombinant proteins
Phenol Red tracer	Sigma-Aldrich	P3532
T7 endonuclease I	New England BioLabs	M0302
Ponceau S Solution	Sigma-Aldrich	P7170
TRIzol™ Reagent	Thermo Fisher Scientific	15596026
7,8-dihydroxyflavone hydrate	Sigma-Aldrich	D5446
SsoAdvanced Universal SYBR Green Supermix	BIO-RAD	1725270
Critical commercial assays
MEGAshortscript T7 Kit	Thermo Fisher Scientific	AM1354
mirVana™ miRNA Isolation Kit	Thermo Fisher Scientific	AM1560
mMESSAGE mMACHINE™ T3 Transcription Kit	Thermo Fisher Scientific	AM1348
RNeasy Mini Kit	QUIAGEN	74104
QIAquick PCR purification Kit	QUIAGEN	28104
Quick Start™ Bradford Protein Assay Kit	BIO-RAD	5000201
NuPAGE™ Large Protein Blotting Kit	Thermo Fisher Scientific	LP0001
TruSeq SmallRNA Sample Prep Kit	ILLUMINA	RS-200-0012
TruSeq Stranded Total RNA Library Prep Kit	ILLUMINA	20020596
iScript™ gDNA Clear cDNA Synthesis Kit	BIO-RAD	1725034
Deposited data
RNA-seq and miRNA-seq of bdnf+/+ and bdnf−/− zebrafish larvae at 24 and 48 hpf	NCBI Sequence Read Archive (SRA)	SUB8904035
Experimental models: Organisms/strains
Zebrafish AB: CRISPR/Cas9 bdnf	Bertolucci/D’Aniello	N/A
Oligonucleotides
Dr_bdnf_gRNA Forward TAGGCGGCGCCCAGGTAGCCCT	This paper	N/A
Dr_bdnf_gRNA Reverse AAACAGGGCTACCTGGGCGCCG	This paper	N/A
Dr_bdnf Forward x PCR screening GAAGAGTGATGACCATCCTG	This paper	N/A
Dr_bdnf Reverse x PCR screening ATGACCTGCTCGAAAGTGTCCG	This paper	N/A
Dr_bdnf Forward x T7 endonuclease I assay GAAGAGTGATGACCATCCTG	This paper	N/A
Dr_bdnf Reverse x T7 endonuclease I assay GTGTACACTATCTGCCCC	This paper	N/A
Dr_nova2 Forward x T7 endonuclease I assay ACAGCCTCCAGTCTCCTGGG	This paper	N/A
Dr_nova2 Reverse x T7 endonuclease I assay TAGCCCACCTTCTGCGGATTGG	This paper	N/A
Primers for qPCR, see Table S5	This paper	N/A
Recombinant DNA
pT7-gRNA vector (Chen lab)	Jao et al., 2013	Addgene # 46759
pCS2-nCas9n (Chen lab)	Jao et al., 2013	Addgene # 47929
Software and algorithms
Guide Design Resources (Zhang lab)	https://zlab.bio/guide-design-resources	N/A
ImageJ Software	Wayne Rasband (NIH)	N/A
ViiA™ 7 Software	Thermo Fisher Scientific	N/A
FASTX Toolkit (Hannon lab)	Affero GPL (AGPL)	N/A
HTseq tool	Anders et al., 2015a, 2015b	http://htseq.readthedocs.io
miRdeep2	Friedländer et al., 2011	http://www.mdc-berlin.de/content/mirdeep2-documentation
DESeq2	Love et al., 2014	https://bioconductor.org/packages/release/bioc/html/DESeq2.html
edgeR	Robinson et al., 2010	https://bioconductor.org/packages/release/bioc/html/edgeR.html
clusterProfiler	Yu et al., 2012	https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html
EthoVision XT Software	NOLDUS	N/A
Prism 5	GraphPad Inc.	N/A
R 3.6.1	www.R-project.org/	N/A
Cosinor	Refinetti et al., 2007	N/A
Other
RIPA Lysis and Extraction Buffer	Thermo Fisher Scientific	89900
Complete Protease Inhibitor Cocktail	MERK	11697498001