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. 2022 Feb 23;101(5):101801. doi: 10.1016/j.psj.2022.101801

Figure 5.

Figure 5

Eugenol improved the expressions of the duodenal tight junction proteins ZO-1, claudin-1 and occludin in Salmonella-infected broilers. (A) WB analysis: the protein bands of critical proteins. (B) WB analysis: the quantification of the protein levels by determining the band intensity and normalized to β-actin band levels. (C) The mRNA expressions of tight junctions (ZO-1, claudin-1 and claudin-2). All experiments were repeated in triplicates. The data are expressed as the Mean ± SE (n = 15). **P < 0.01, *** P < 0.001 vs. Control. # P < 0.05, ## P < 0.01, ### P < 0.001 vs. SL1344 infection.