Fig. 4.

Arc is regulated by the DNMT3A/miR-377-5p pathway. A Arc mRNA expression after DNMT3A knockdown or overexpression quantified using RT-PCR. B Arc protein expression after DNMT3A knockdown or overexpression identified using western blot analysis, and the full-length blots/gels are presented in Figure S4. C the changes of DNMT3A protein expression after DNMT3A knockdown or overexpression; D Arc protein expression quantified using western blot analysis. E targeted regulation of miR-377-5p on Arc identified through luciferase reporter assay. F miR-377-5p expression levels after the introduction of miR-377-5p mimcs or inhibitor quantified using PR-PCR; G Arc mRNA expression levels quantified using RT-PCR; H the changes of Arc protein expression after the introduction of miR-377-5p mimcs or inhibitor identified using western blot analysis, and the full-length blots/gels are presented in Figure S5; I Arc protein levels detected using western blot analysis. Each value represents the mean ± SD for n = 3. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. DEX, dexmedetomidine; KD, knockdown; OE, overexpression; WT, wild-type; MT, mutant-type