|
Antibodies
|
| Rat Anti-ATE1 |
EMD-Millipore |
Cat# MABS436, clone 6F11 |
| Mouse anti-β-actin |
Sigma Aldrich |
Cat# A1978 |
| Mouse anti-GFP |
Roche |
Cat# 11814460001 |
| Mouse anti-HA |
Thermo Fisher |
Cat#26183 |
| Rabbit anti-HIF1α |
Abcam |
Cat# ab179483 |
| Mouse anti-HIF1α |
R&D systems |
Cat# MAB1536 |
| Rabbit anti-hydroxyl HIF1α |
Cell Signaling |
Cat# 3434 |
| Rabbit anti-hydroxyl HIF1α |
Rockland |
Cat# 100-401-A25 |
| Rabbit anti-VHL |
Thermo Fisher |
Cat#PA5-27322 |
| Rat anti-tubulin |
Novus Biologicals |
NB600-506SS |
| Mouse anti-tubulin |
Sigma-Aldrich |
Cat# T5201 |
| Anti-PHD2 |
Santa Cruz |
Cat# SC271835 |
| Mouse anti-UBR1 |
Santa Cruz |
Cat#sc515753 |
| Rabbit anti-R-HIF1 α |
GenScript |
Custom ordered |
|
Bacterial and virus strains
|
| TOP10 Chemically Competent E.Coli |
Thermo Fisher |
Cat#C404010 |
|
Biological samples
|
|
|
| N/A |
|
|
|
Chemicals, peptides, and recombinant proteins
|
| Polyethyleneimine |
Sigma-Aldrich |
Cat#408727; CAS: 9002-98-6 |
| Cycloheximide |
Sigma-Aldrich |
Cat#01810-1G |
| L-Ascorbic acid 2-phosphate |
Sigma-Aldrich |
Cat# A8960 |
|
Critical commercial assays
|
| SuperSignal Femto Chemiluminescence Kit |
ThermoFisher |
Cat# 34096 |
| Quick-RNA Miniprep Kit |
Genesse Scientific |
Cat #: 11-328 |
| Superscript first-strand synthesis system for RT-PCR |
Invitrogen |
Cat#: 11904-018 |
| SSOAdvanced Universal SYBR green Supermix |
Biorad |
Cat#: 1725271 |
| XF Glycolysis Stress Test Kit |
Agilent |
Cat# 103020-100 |
| CellTiter-Glo Luminescent assay |
Promega |
Cat # G7575 |
| Glucose Uptake Cell-Based Assay Kit |
Cayman |
Cat#600470 |
| Restore™ Western Blot Stripping Buffer |
ThermoScientific |
Cat# 21059 |
| Western blot Blocking buffer |
Roche |
Cat#1096176001 |
|
Deposited data
|
| N/A |
|
|
|
Experimental models: Cell lines
|
| MEF |
Gift from Dr. Anna Kashina (Kwon et al., 2002) |
https://www.ncbi.nlm.nih.gov/pubmed/12098698
|
| MEF ATE1-KO |
Gift from Dr. Anna Kashina (Kwon et al., 2002) |
https://www.ncbi.nlm.nih.gov/pubmed/12098698
|
| HEK293T |
ATCC |
ATCC CRL-11268 |
| HFF |
Gift from Dr. John Murray (Indiana University) |
N/A
|
| UOK111 |
Gift from Dr. W. Marston Linehan (National Cancer Institute) |
N/A
|
| PC-3 and PC3-ML |
Gift from Dr. Kerry Burnstein (University of Miami) |
N/A
|
|
Experimental models: Organisms/strains
|
| N/A |
|
|
|
Oligonucleotides
|
|
See
Supplemental Table S2
for a list of oligonucleotides.
|
|
|
|
Recombinant DNA
|
| Delta R8.2 |
N/A |
Addgene 12263 |
| VSV-G |
Stewart et al., 2003
|
Addgene 8454 |
| pQC-XIG |
N/A |
Addgene 26826 |
| Mouse HIF1A coding sequence ([NM_010431.2]) |
|
Vectorbuilder |
| pQC-link-M-HIF1α-HA-XIG |
This study |
N/A
|
| pQC-link-G-HIF1α-HA-XIG |
This study |
N/A
|
| pQC-link-R-HIF1α-HA-XIG |
This study |
N/A
|
| pQC-link-E-HIF1α-HA-XIG |
This study |
N/A
|
| pQC-link-M-HIF1αPAPG-HA-XIG |
This study |
N/A
|
| pQC-link-G-HIF1αPAPG-HA-XIG |
This study |
N/A
|
| pQC-link-R-HIF1αPAPG-HA-XIG |
This study |
N/A
|
| pQC-link-E-HIF1αPAPG-HA-XIG |
This study |
N/A
|
|
|
|
|
Software and algorithms
|
| R |
|
http://www.R-project.org/
|
| cBioPortal for Cancer Genomics |
|
https://www.cbioportal.org/
|
| T-COFFEE/M-Coffee |
|
http://tcoffee.crg.cat
|
| CFX 3.1 manager |
Bio-Rad |
http://www.bio-rad.com/en-us/sku/1845000-cfx-manager-software?ID=1845000
|
| FCS Express 7 |
De Novo Software |
https://denovosoftware.com/academic-research/
|
| ImageQuant TL software pack |
Cytiva |
https://www.cytivalifesciences.com/en/us/shop/molecular-biology/nucleic-acid-electrophoresis--blotting--and-detection/molecular-imaging-for-nucleic-acids/imagequant-tl-8-2-image-analysis-software-p-09518
|
|
Other
|
| N/A |
|
|
