TABLE 3.

Percent penetration of AmB and Flu into cardiac vegetations in rabbits treated intravenously with either AmB at 1.0 mg/kg per day or Flu at 85.2 mg/kg in divided doses for 4 days

Drug tested	Mean trough serum drug concna (μg/ml) ± 1 SD	Mean drug concn (μg/ml) ± 1 SD in:		% Penetration of drug into cardiac vegetationsc
		Cardiac vegetations	Vegetations of controlsb
AmB	0.43 ± 0.03	0.21 ± 0.03	0.18 ± 0.02	6.98
Flu	34.38 ± 6.51	2.02 ± 1.33	Not detectable	5.88

^aTrough levels were measured from serum samples that were collected from infected rabbits 24 h after they received their last dose of AmB or 12 h after they received their last dose of Flu. Cardiac vegetations were collected from sacrificed animals immediately thereafter. 

^bVegetation controls are vegetations collected from untreated, infected rabbits that were dipped in solutions of AmB at 0.45 μg/ml or Flu 35 at μg/ml and then washed with D₅W before the vegetations were homogenized. They are used to determine the concentration of drug that coats the vegetations. The lower limits of sensitivity of the AmB and Flu bioassays were 0.1 and 0.5 μg/ml, respectively. 

^cPercent penetration was calculated from the following formula: [(drug concentration in cardiac vegetations − drug concentration in vegetation controls)/trough serum drug concentration] × 100.