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. 2022 Mar 22;17:1309–1322. doi: 10.2147/IJN.S356453

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© 2022 Si et al.

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In vivo anti-tumor efficacy of drugs-loaded hydrogels after single injection to the vicinity of the tumors of human osteosarcoma xenografts. (A) Tumor volume. (B) The body weights of mice during the treatments. (C) The tumor weights from the mice sacrificed. (**p<0.01, ***p<0.001). (D) Individual tumor growth curves after treatment. (E) Ex vivo histological H&E staining and TUNEL assay analyses of tumor sections after various treatments. Nuclei were stained blue, and extracellular matrix and cytoplasm were stained red in H&E staining. Green fluorescence indicated apoptotic cells in TUNEL analysis.