FIG. 2.
(a) Unbinding rates computed by InfrMetaD for the potential in Fig. 1(a) given in μs−1. The log of the rates increases linearly with force (given in pN), apparently following Bell’s law. The fit parameters are k0 = 21.90 and Δx‡ = 6.86 (R2 = 0.99). (b) Rates computed by InfrMetaD plotted against the energy barrier computed by WTMetaD exhibit Arrhenius behavior. (c) The transition distances computed from FE calculations shrink with applied force as predicted by extended Bell’s theory,42 meaning that not all assumptions of Bell’s law are true. “Theory” values are the shift in the analytical potential with force. (d) The computed energy barrier to unbinding decreases linearly as higher forces are applied in accordance with the assumption going into Bell’s law. Ubarrier is the analytical barrier height.