FIG. 9.

(a) Unbinding rates computed from InfrMetaD show an overall slip bond but a poor fit to Bell’s law. The fit parameters used are Δrc^‡ = 2.1 Å and k⁰ = 43 s⁻¹ (R² = 0.59). This fitted Δrc^‡ is not close to the transition distance observed in the FES estimates. (b) Estimation of free energy surfaces obtained from WTMetaD shows a rough landscape for ligand escape at small force as well as a transition to a tilted landscape favoring unbinding from F = 12 pN and above. Surfaces for other forces can be found in Fig. S8.