Figure 8. Lateral disc puncture with nerve exposure induced increased Iba1+ macrophages/microglia in the ipsilateral spinal nerves.
(a) Immunostaining of axial spine sections showed abundant Iba1+ macrophages in the ipsilateral nervescompared to the contralateral nerve on POD 7. Black arrows point to nerves. Scale bar=50 μm. (b) Quantitative analysis showed more than a 2-fold increase in Iba1+ cells in the ipsilateral nerve vs. the contralateral side. **p<0.01, n=3 per group, unpaired t-test.
