Representative confocal images of C. albicans-S. mutans, C. albicans-S. gordonii, and C. albicans-S. mutans–S. gordonii biofilms cultured in media supplemented with 1% sucrose. Gray, green, red, and blue colors indicate C. albicans, S. mutans, S. gordonii, and extracellular polysaccharides (EPS)-matrix, respectively. Adapted with permission from Wan et al. (2021) Cross-Kingdom Cell-to-Cell Interactions in Cariogenic Biofilm Initiation. Journal of Dental Research, Vol. 100(1) 74–81. Copyright © International & American Associations for Dental Research 2020