Figure 2: CD69+ cells are CD3+ T cells and express CCR7.

Triple labeling using (a) CCR7 (red); (b) CD3 (green); and (c) CD69 (magenta) of 4 lesional biopsies per treatment group was performed to identify CD69+ T cells. (d) CCR7 was found on almost all immune cells and overlapped with CD3 as see in yellow. (e) CD69 was found to overlap completely CD3 (white) identifying them as T cells. (f) Further overlay of all three markers revealed overlap of CCR7, CD3, and CD69 (white).